ProtocolGPT

ProtocolGPT is an AI-powered tool that has been specifically designed to guide users in the creation of biology protocols.

Overview

ProtocolGPT is an AI-powered tool that has been specifically designed to guide users in the creation of biology protocols. ProtocolGPT has been trained on a vast database of biology protocols, enabling it to understand the structure and content of a wide range of experimental procedures.

ProtocolGPT training currently covers a wide range of experimental protocols such as DNA extraction, RNA isolation, PCR amplification, western blotting, ELISA, flow cytometry, and many more. These protocols are commonly used in the fields of molecular biology, biochemistry, and cell biology.

The training of ProtocolGPT is based on a combination of natural language processing and machine learning techniques, which enables it to understand the language used in scientific protocols and generate accurate and detailed instructions for experimental procedures.

The addition of new protocols to ProtocolGPT is an ongoing process, and we are constantly updating our training data to improve the accuracy and comprehensiveness of our models. If you have a protocol that you think would be useful to add to our training data, please do not hesitate to contact us.

Overall, ProtocolGPT training provides a powerful tool for scientists and researchers to streamline their experimental procedures and reduce errors caused by misinterpretation or miscommunication of experimental protocols. With ProtocolGPT training, scientists can focus more on the science and less on the details of experimental procedures.

Protocols

Below is a comprehensive list of the protocols that are covered by ProtocolGPT training. However, if you have a specific protocol that is not listed below, do not hesitate to suggest it to us at https://www.labii.com/contact-us. Please note that even if your protocol is not explicitly listed, it may still work since ProtocolGPT is trained based on existing Large Language Models (LLMs).

Antibody

  • Western blot

  • Immunohistochemistry, IHC Protocol

  • Immunoprecipitation (IP) Protocol

  • IF Protocol, Immunofluorescent Staining Protocol

  • FACS Protocol, Flow Cytometry Protocol

  • Chromatin Immunoprecipitation

  • CUT&RUN

Biochemistry

  • ELISA (Enzyme-linked immunosorbent assay)

  • Northern blot

  • Southern blot

  • RNA interference (RNAi)

  • Isoelectric focusing

  • MALDI-TOF (Matrix-assisted laser desorption/ionization time-of-flight)

  • FRET (Fluorescence resonance energy transfer)

  • SPR (Surface plasmon resonance)

  • Mass spectrometry

  • X-ray crystallography

  • NMR (Nuclear magnetic resonance)

  • DSC (Differential scanning calorimetry)

  • ITC (Isothermal titration calorimetry)

  • SEC (Size-exclusion chromatography)

  • HPLC (High-performance liquid chromatography)

  • Affinity chromatography

  • Ion exchange chromatography

  • Reverse-phase chromatography

  • Immunoaffinity chromatography

  • FPLC (Fast protein liquid chromatography)

  • BRET (Bioluminescence resonance energy transfer)

  • Protein microarray

  • Yeast two-hybrid

  • Site-directed mutagenesis

  • CRISPR-Cas9 gene editing

  • Metabolic labeling

  • Pulse-chase experiment

  • Liposome fusion assay

  • Lipid rafts isolation assay

  • Glycosylation analysis

  • Glycan microarray

  • Enzyme kinetics

  • Protein folding kinetics

  • Fluorescence anisotropy

  • Circular dichroism

  • UV-Vis spectroscopy

  • Fluorescence spectroscopy

  • Confocal microscopy

  • Fluorescence microscopy

  • Electron microscopy

  • Scanning electron microscopy (SEM)

  • Transmission electron microscopy (TEM)

  • Atomic force microscopy (AFM)

  • Optical tweezers

  • Laser trap

  • Patch clamp technique

  • Microinjection

  • siRNA (small interfering RNA)

  • miRNA (microRNA)

  • shRNA (short hairpin RNA)

  • Ribosome profiling

  • RNA sequencing (RNA-seq)

  • ChIRP (Chromatin Isolation by RNA Purification)

  • PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) Ribo-seq (Ribosome profiling by deep sequencing)

  • TALEN (Transcription activator-like effector nucleases)

  • Zinc finger nucleases (ZFNs)

Cell biology

  • 3d cell culture

  • Apoptosis assays

  • Cell adhesion assay

  • cell culture

  • Cell cycle analysis

  • Cell migration assays

  • Cell proliferation assays

  • Cell viability assays

  • Co-culture

  • Fluorescence-activated cell sorting (FACS)

  • Glycomics

  • Invasion assay

  • Laser capture microdissection (LCM)

  • Lipidomics

  • Live cell imaging

  • Metabolomics

  • Organoid culture

  • Organotypic culture

  • Proteomics

  • Transduction assay

  • Transwell assay

  • Wound healing assay

Microbiology

  • Gram staining

  • Acid-fast staining

  • Capsule staining

  • "Spore staining

  • "Flagella staining

  • Microbial culture

  • Microbial identification

  • Antimicrobial susceptibility testing

  • Disk diffusion assay

  • Broth microdilution assay

  • E-test

  • Kirby-Bauer method

  • MIC (Minimum inhibitory concentration) assay

  • Agar dilution assay

  • Colony counting

  • Serial dilution

  • Spread plate method

  • Pour plate method

  • Streak plate method

  • Biofilm assay

  • Quorum sensing assay

  • Microbial growth kinetics

  • Optical density measurement

  • CFU (Colony-forming units) measurement

  • Microbial transformation

  • Electroporation

  • Chemical transformation

  • Conjugation

  • Transduction

  • RNA interference (RNAi)

  • Bacterial mutagenesis

  • Transposon mutagenesis

  • Directed mutagenesis

  • Phage display

  • Phage therapy

  • Microbial fermentation

  • Metagenomics

  • Microbial ecology

  • FISH (Fluorescent in situ hybridization)

  • Immunofluorescence microscopy

  • Confocal microscopy

Molecular biology

  • Gel electrophoresis

  • SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis)

  • PCR (Polymerase chain reaction)

  • Cloning

  • RT-PCR (Reverse transcription polymerase chain reaction)

  • qPCR (Quantitative PCR)

  • DNA sequencing (DNA-seq)

  • RNA sequencing (RNA-seq)

  • Sanger sequencing

  • Next-generation sequencing (NGS)

  • Microarray

  • Isoelectric focusing

  • Plasmid isolation

  • Restriction enzyme digestion

  • Ligation

  • Gibson assembly

  • Golden Gate assembly

  • RNA interference (RNAi)

  • cDNA synthesis

  • Gene expression analysis

  • Cloning

  • Overexpression

  • Knockout

  • Gene silencing

  • Lipofection

  • In vitro transcription/translation

  • Protein purification

  • Virus

  • DNA Extraction

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