ELN for Dose Response Curve
Labii Electronic Lab Notebook (ELN) for Dose Response Curve is a specialized solution designed to simplify and accelerate dose-response experimentation and analysis with automated 4-parameter logistic
Overview
Labii Electronic Lab Notebook (ELN) for Dose Response Curve is a specialized solution designed to simplify and accelerate the process of dose-response experimentation and analysis. With its dedicated Dose Response Curve widget, Labii provides a structured layout for inputting concentrations and responses, automates the application of a 4-parameter logistic (4PL) model for curve fitting, and delivers precise standard curve generation with minimal manual effort. This robust tool enhances experimental accuracy and efficiency, making it ideal for researchers working with pharmacological assays, ELISAs, and other bioassays where modeling dose-response relationships is critical.
The dose-response curve is a critical tool in understanding how varying levels of exposure to a stressor affect an organism. It's commonly used in different fields, from pharmaceuticals to environmental studies, to determine safe levels of exposure or dosage.
Watch our demo video to see the Dose Response Curve widget in action, or explore the detailed widget documentation.
Installation
To install the Electronic Lab Notebook (ELN) for Dose Response Curve application:
Navigate to Settings and select Applications
Click Add application and select Add from a template
Choose Electronic Lab Notebook (ELN) for Dose Response Curve from the template list
If the application is not available in the list, it may already be installed in your workspace.
Design
The application consists of two tables: "drc" and "protocol".
Table "drc":
Columns:
date_start (required)
date_due
signature_status
Sections:
DRC Workflow: A flowchart widget that guides the user through the experiment steps.
Experiment Overview
Experiment Procedure
Dose Response Curve: A Dose Response Curve widget that helps in preparing layouts and analyzing data.
Other Files: A Files widget for attaching additional files to the experiment.
Experiment Signers
General Usage
The general usage of the application includes:
Creating new records: Users can create new Dose Response Curve records by filling in the required fields, such as the start date. They can also provide additional information in the experiment overview and procedure sections.
Managing records in the table list view: Users can navigate through the list view of Dose Response Curve records and perform actions like editing, deleting, or sorting the records.
Viewing record details: Users can access the detailed view of a specific Dose Response Curve record to see all the sections and widgets associated with it.
Special Usage
The Labii ELN for Dose Response Curve application offers a specialized workflow and Dose Response Curve widget that simplifies conducting and analyzing dose-response experiments.
Workflow
The DRC Workflow section provides a step-by-step flowchart guiding users through the various stages of the experiment, from providing an overview to conducting the experiment, preparing layouts and data, performing the analysis, and signing the experiment.
Dose Response Curve Widget
Labii's Dose Response Curve widget transforms complex dose-response curve generation into a streamlined, user-friendly workflow. The widget supports flexible data entry with clearly defined rules for concentrations, blanks, and controls. It automatically calculates background-corrected averages, applies a robust Four Parameter Logistic (4PL) model, and visualizes the results with interactive plots and confidence intervals.
The process involves three main steps:
Prepare Layout
Set up the 96-well plate layout with concentration values:
Input data manually or import from files
Mark concentrations using specific conventions
Define blank wells and positive controls
Organize your dose-response experimental design
Prepare Response Data
Enter the response data into the 96-well plate:
Input measured responses for each well
Similar data entry methods as layout preparation
Empty wells can be excluded from analysis
Supports various data import formats
Automated Analysis
The widget automatically performs comprehensive analysis:
Baseline Calculation: Generates average responses of blanks and positive controls to establish baselines
Background Correction: Subtracts background values and applies data transformations as needed
4PL Curve Fitting: Fits a four-parameter logistic (4PL) curve using the Levenberg–Marquardt algorithm to characterize the dose-response relationship
Visualization: Plots the dose-response curve with data points, estimated plots, and confidence intervals
Key Parameters: Calculates EC50 (or IC50), Hill slope, minimum, and maximum responses
Advanced Tuning Options: Fine-tune settings including response value transformations, X-axis scale adjustments, and initial parameters for the algorithm to improve curve fitting with challenging datasets. Intelligent warnings help ensure precision even with complex data patterns.
While the algorithm aims for the best fit, it might not always find the optimal solution with complex data patterns. Use the advanced tuning options to adjust initial guesses or try different settings when working with challenging datasets.
Includes All Features of Labii ELN for Production
Labii ELN for Dose Response Curve inherits all the robust capabilities of the ELN for Production, including:
Structured Data Layouts: Organized templates for consistent documentation
Automated Templates: Pre-configured workflows to accelerate experimentation
Version Control: Track all changes and maintain complete revision history
Access Permissions: Granular control over who can view and edit data
Full Audit Trails: Complete documentation of all activities for compliance
Regulatory Compliance: Meets FDA 21 CFR Part 11 requirements
Electronic Signatures: Secure signing workflows for data approval
This ensures your dose-response curve documentation meets both scientific and compliance demands—without compromising flexibility or ease of use.
Related Applications
Explore other specialized ELN solutions from Labii:
Conclusion
Labii ELN's Electronic Lab Notebook (ELN) for Dose Response Curve provides researchers with a comprehensive solution for conducting dose-response experiments and analyzing the obtained data. Through the specialized workflow and Dose Response Curve widget, researchers can streamline their experimental workflows, ensure precision and accuracy, and enhance the overall reliability of scientific investigations. With automated 4PL curve fitting, interactive visualizations, and full regulatory compliance features, Labii makes dose-response analysis accessible and efficient for pharmacological assays, ELISAs, and other critical bioassays.
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