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On this page
  • Overview
  • How the assay works
  • Use case
  • Configuration
  • Interface
  • Analysis Workflow
  1. Widgets
  2. Section Widgets
  3. Biology
  4. Bioinformatics

QUiCKR Analysis

QUiCKR utilizes CRISPR kinetic rates for precise quantification through a streamlined process—from sample prep and plate transfer to fluorescent readout and cloud-based instant results.

PreviousBioinformaticsNextCRISPResso2

Last updated 3 months ago

Overview

The QUiCKR Analysis widget allows users to seamlessly upload files to Labii Experiment and obtain instant quantification of genetic modifications for Cell and Gene Therapies. With , researchers can file IND applications 10 months sooner and save 30% on testing costs, accelerating lead optimization from 360 days to just 70 days. This high-throughput, automated solution requires no new instruments and has already analyzed 30,000+ reactions. Featuring a 20-minute sample-to-results workflow, QUiCKR utilizes CRISPR kinetic rates for precise quantification through a streamlined process—from sample prep and plate transfer to fluorescent readout and cloud-based instant results. Faster insights mean earlier therapy sales and quicker market entry.

How the assay works

The workflow consists of serial dilutions of DNA samples, followed by transfer of the dilution series to the QUiCKR plate. The plate is run on standard fluorescence readers and the output file is uploaded to the QUiCKR platform for instant analysis.

Use case

  • Perform QUiCKR Analysis.

Configuration

For the widget to function, specific authentication and mapping information

  • Authentication Information - QUiCKR API authentication information.

    • Bearer Token - Bearer Token or any other authorization string. Bearer Tokens are the predominant type of access token used with OAuth 2.0. For example: Bearer eyJraWQiOiIy

    • Backend - QUiCKR API backend URL.

  • Columns - Map the columns to the QUiCKR API columns.

    • Date

    • Project

    • Kit

    • Machine

    • Samples

    • Has Control

Interface

The QUiCKR Analysis interface provides a seamless and intuitive experience for quantifying genetic modifications. By default, users see a file selection area, allowing them to easily upload their experiment data. Once a file is selected and analyzed, the interface dynamically updates to display comprehensive summary results, including indel quantification and a detailed layout heat chart for visualizing modifications. This streamlined workflow ensures users can quickly interpret their data with high accuracy, leveraging QUiCKR’s advanced analytics for faster decision-making in Cell and Gene Therapies.

Analysis Workflow

The QUiCKR Analysis Workflow ensures a seamless and efficient process for quantifying genetic modifications. Users start by selecting a file, which undergoes a format check to ensure compatibility. Once validated, a new experiment and run are created in QUiCKR, followed by file upload to QUiCKR for processing. The system then performs automated analysis, generating instant results that include summary insights, indel quantification, and a layout heat chart for easy interpretation. This streamlined workflow accelerates data-driven decisions, enabling faster and more accurate genetic analysis.

  1. Select File – Users begin by selecting a file for analysis.

  2. File Format Check – The system verifies the file format to ensure compatibility.

    1. If the check passes, proceed to the next step.

  3. Create Experiment – A new experiment is created in QUiCKR.

  4. Create Run – A run is generated within the experiment.

  5. Upload File to QUiCKR – The selected file is uploaded to QUiCKR for processing.

  6. Perform Analysis – QUiCKR conducts the genetic modification quantification.

  7. Display the Results – The analyzed data, including summary results, indel quantification, and layout heat chart, is presented to the user.

QUiCKR