Dose Response Relationship

Dose Response Curve

Overview

The dose–response relationship, or exposure–response relationship, describes the change in effect on an organism caused by differing levels of exposure (or doses) to a stressor (usually a chemical) after a certain exposure time, or to a food. This may apply to individuals (e.g., the dose makes the poison: a small amount has no significant effect, a large amount is fatal), or to populations (e.g.: how many people or organisms are affected at different levels of exposure).

Studying dose response, and developing dose–response models, is central to determining "safe", "hazardous" and (where relevant) beneficial levels and dosages for drugs, pollutants, foods, and other substances to which humans or other organisms are exposed. These conclusions are often the basis for public policy.

The U.S. Environmental Protection Agency has developed extensive guidance and reports on dose-response modeling and assessment, as well as software. The U.S. Food and Drug Administration also has guidance to elucidate dose-response relationships during drug development.

A dose–response curve is a simple X–Y graph relating the magnitude of a stressor (e.g. concentration of a pollutant, amount of a drug, temperature, intensity of radiation) to the response of the receptor (e.g. organism or population under study). The response may be a physiological or biochemical response, or even death (mortality), and thus can be counts (or proportion, e.g., mortality rate), ordered descriptive categories (e.g., severity of a lesion), or continuous measurements (e.g., blood pressure).[4] A number of effects (or endpoints) can be studied, often at different organizational levels (e.g., population, whole animal, tissue, cell).

-- from https://en.wikipedia.org/wiki/Dose%E2%80%93response_relationship

Labii has developed widgets to perform dose-response relationship analysis:

Category

Widget

Type

Description

DRC

Dose Response 96-Well

Section

Perform dose-response relationship analysis

Dose Response 96-Well

This widget can:

  • Design layout based on 96-well plate

  • Transform (normalize) the data with provided formula

  • Perform dose response curve fit

  • Calculate EC50/IC50, min, max, hill slope

  • Draw dose response curve

Design the plate

Click the LABEL tab to navigate to 96-well layout for labels. You can customize the compound, control and dose at this tab.

The Label interface of Dose response 96-well
  • Labels goes to first row and first column. Specifically, does label goes to the first row.

  • The label and value of control is optional. The control label can be defined during the analysis.

  • You do not need to use all wells. The blank wells will be block out.

You can drag and drop or click the cloud icon to load content quickly.

Upload the value

Click the VALUE tab to switch to 96-well layout for values. You can upload the values in this tab.

The Value interface of Dose response 96-well

You can drag and drop or click the cloud icon to load content quickly.

Perform analysis

Click the ANALYSIS button to prepare the analysis. This requires the label and value provided. Click the Submit to start analysis. It usually take a few seconds to perform the analysis.

  • Data Transform Formula - Use this formula to transform or normalize your data. The widget will replace your value accordingly.

    • VALUE - The data point

    • UPPER - The average value of Upper Control

    • LOWER - The average value of Lower Control

  • Label (Control) - The label of the Control. For example "Control".

  • Label (Upper Control) - The label of the Upper Control. For example "Upper"

  • Label (Lower Control) - The label of the Lower Control. For example "Lower"

The Label for the controls are case sensitive. The mismatch will cause the error of "Control not found".

Click "Add Parameters" to provide compound specific parameters.

  • Compound - The label of the compound. It have to be identical to the label design in the 96-well

  • Hill Slope - The value of hill slope you want to customize.

  • maxfev - The maximum number of calls to the function during the curve fit. Default to 1000, max to 10000. Increase this value when you see the error of "Reach maxfev limit.'

Compound specific parameters

The results

If the analysis is successful, you shall see a list of results.

Mean of controls

This give you the average value of each control.

Data of response

This gives you the formatted data for dose response curve fit. The data display the columns of compound, dose, response, response_transformed. Each duplicates are displayed in one row. The blocked out data point will not be included.

Data of fit

This gives the calculated value for top, bottom, hill slope, ec50 after fit.

Dose response curve

A new picture will be created and saved to your files. The created the curve file is downloadable.